C（3〜6か月） <a href='https://brc.riken.jp/mus/pcr06449'>Genotyping protocol -PCR-</a> Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> H3mmT KO, B6-H3mmT EN, H3mmT knockout mouse H3mmT KO, B6-H3mmT EN, H3mmT knockout mouse true Sperm specific histone H3 variant, H3mmT gene knockout mice. A 16 bp including ATG was deleted. Homozygous mutant males are infertile. 大阪大学微生物病研究所・上田　潤先生・山縣一夫先生、九州大学大学院医学研究院・前原一満先生、原田哲仁先生、大川恭行先生（2013）。C57BL/6受精卵にCas9とgRNA発現プラスミドを導入することにより作出。ホモノックアウトマウスはH3mmTタンパクを発現していない（ウェスタンブロットにより確認）。Off-target解析は未実施。 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. <br>研究成果の公表にあたって謝辞の表明を必要とする。<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。 RBRC06449 山縣　一夫 精巣特異的新規ヒストンH3バリアントH3mmTノックアウトマウス。CRISPR/Casシステムを用いて、開始コドンを含む16塩基を欠損する。ホモノックアウト雄マウスは不妊。 C57BL/6-H3t<em1Osb> C57BL/6-H3t<em1Osb> Heterozygote x Wild-type [C57BL/6NCrSlc] Heterozygote x Wild-type [C57BL/6NCrSlc] Kazuo YAMAGATA C (3-6 months) [hCas9 (addgene ID41815; CMV IE promoter, SV40 nls, human codon optimized Cas9*, neo*, TK pA), gRNA Cloning vector (addgene ID41824; human U6 polymerase III promoter, neo)] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. Developed by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University and Kazumitsu Maehara, Akihito Harada and Yasuyuki Ohkawa, Graduate School of Medical Sciences, Kyushu University in 2013. Generated by injecting Cas9 and gRNA (GCAAGGGAGGCGGACGATTCAGG) expression plasmids into the C57BL/6 fertilized eggs. Off-target analysis was not examined.